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Anti-oxidant and Anti-inflammatory effects of Usnea diffracta Vain. extracts

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KMID : 1161820210060010001
ÃÖ¿¬ ( Choi Yeon ) - Mind Healing Institute

±èÁ¾µÎ ( Kim Jong-Du ) - Dongbang Culture University Department of Naturopathic

Abstract

º» ¿¬±¸´Â ¼Û¶ó ÃßÃâ¹°ÀÌ ÇǺΰü·Ã Áúȯ¿¡ Áß¿äÇÑ ±â´É¼º ¼ÒÀç·Î¼­ÀÇ °¡´É¼ºÀ» È®ÀÎÇϱâ À§ÇØ Ç×»êÈ­¿ÍÇ׿°È¿°ú¸¦ È®ÀÎÇÏ¿´´Ù. ±× °á°ú DPPH ¿Í ABTS ¶óµðÄà ¼Ò°Å´É È°¼º¿¡¼­ 50% ¿¡Åº¿Ã, 70%¿¡Åº¿Ã, 95% ¿¡Åº¿Ã ÃßÃâ¹°¿¡¼­ ³ôÀº Ç×»êÈ­ È°¼ºÀ» È®ÀÎÇÏ¿´°í, ºÎºÐÀûÀ¸·Î 70%¿¡Åº¿Ã, 95% ¿¡Åº¿Ã ÃßÃâ¹°¿¡¼­´Ù¸¥ ÃßÃâ¹°°ú ´Þ¸® ³·Àº ³óµµ¿¡¼­µµ ³ôÀº Ç×»êÈ­ È°¼ºÀ» ³ªÅ¸³¿À» È®ÀÎÇÏ¿´´Ù. ³ôÀº Ç×»êÈ­ È°¼ºÀ»º¸ÀÌ´Â 70% ¿¡Åº¿Ã, 95% ¿¡Åº¿Ã ÃßÃâ¹°¿¡ ´ëÇØ ÇǺΰ¢Áú ¼¼Æ÷ÀÎ HaCaT ¼¼Æ÷ÁÖ¸¦ ÀÌ¿ëÇØ ÇǺο°Áõ ¿ÏÈ­È¿´ÉÀ» °ËÁõÇÑ °á°ú. 70% ¿¡Åº¿Ã 95% ¿¡Åº¿Ã ÃßÃâ¹°À» ³óµµº° 󸮽à TNF-¥á¿Í IFN-¥ã¿¡ ÀÇÇØ Áõ°¡µÈ»çÀÌÅäÄ«ÀÎÀÎ CCL17°ú CCL22ÀÇ ¼öÄ¡°¡ ³óµµÀÇÁ¸ÀûÀ¸·Î °¨¼ÒµÇ´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù. °á·ÐÀûÀ¸·Î ¼Û¶ó70% ¿¡Åº¿Ã, 95% ¿¡Åº¿Ã ÃßÃâ¹°Àº ÇâÈÄ ÇǺÎÁúȯ¿¡ °ü·ÃµÈ ±â´É¼º ¼ÒÀç ¹× ¹Î°¨¼º ÇǺΰü·Ã È­ÀåÇ°¼ÒÀç·Î¼­ »ç¿ëµÉ °¡´É¼ºÀÌ »ç·áµÈ´Ù,

This study investigated antioxidant and anti-inflammatory in order to confirm the possibility of functional materials that can improve skin diseases using Usnea diffracta Vain. extracts. As a result of measuring DPPH and ABTS free radical scavenging activities, it was confirmed that 50%% ethanol, 70% ethanol, and 95% ethanol extracts had superior antioxidant activity compared to other solvent extracts. In particular, in the case of 70% ethanol, 95% ethanol extract, it was confirmed that the antioxidant ability was excellent even at a low concentration compared to other extracts. As a result of confirming the efficacy of reducing skin inflammation against 70% ethanol, 95% ethanol extract using the HaCaT cell line, it was confirmed that the protein level of the CCL17 and CCL22 cytokine increased by TNF-¥á and IFN-¥ã decreased dosedependent, respectively. In conclusion, the 70% ethanol, 95% ethanol extract of Usnea diffracta Vain. is likely to be used as a functional material related to skin diseases in the future.
KeyWords
¼Û¶ó, Ç×»êÈ­, Ç׿°, ½ÎÀÌÅäÄ«ÀÎ, ÇǺÎÁúȯ
Usnea diffracta Vain., anti-oxidant, anti-inflammatory, cytokine, skin diseases
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ÇмúÁøÈïÀç´Ü(KCI)